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KMID : 1812020160220020346
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Journal of Neurogastroenterology and Motility
2016 Volume.22 No. 2 p.346 ~ p.347
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The New Calcium Imaging Trend (Front Cell Neurosci 2015;9:436)
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Abstract
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Calcium imaging is a technique for intracellular calcium measurement, and it is important to understand the neural and glial functions. Genetically encoded Ca2+ indicators have been used extensively in many body systems to detect Ca2+ transients associated with neuronal activity. The aim of this article is to utilize a number of cell-specific promoters that express the Ca2+ indicator GCaMP3 in different classes of neurons and glia to determine their effectiveness in measuring activity in enteric neural networks during colonic motor behaviors. The authors found that the activity in glial networks appeared to follow neural activity but continued long after neural activity had waned. With these new tools an unprecedented level of detail can be recorded from the enteric nervous system (ENS) with minimal manipulation of tissue. These techniques can be extended in order to better understand the roles of particular enteric neurons and glia during normal and disordered motility.
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KEYWORD
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Journal Club
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